Category Archives: Molecular Diagnostics

Gentaur VOLTRAN Viral Load Detection System

bba5585d-ML_STARlet_IVD_seitlich“Perfect combination of unrivalled technology and the quality”

Minimize the hands on time. Standardize your assay. Get the accurate quantification. RTA now presents the perfect combination of the proven quality Gentaur Real Time PCR Kits and the best in class instrumentation Hamilton MICROLAB STARLET(CE-IVD) and BioRad CFX96TM (CE-IVD) for your routine detection of HBV, HCV, HIV-1 and CMV nucleic acids.
Gentaur VOLTRAN® Viral Load Detection System offers everything you need for a fast, productive and reliable operation:
• Compliance with EU IVD 98/79/EC
• Automated nucleic acid extraction (magnetic bead) and PCR Setup
• 96 sample capacity, build-in and handheld barcode scanner
• Inhibitor-free nucleic acid checked by internal control added during extraction
• Leading pipetting technology of HAMILTON based on 50 years of expertise in
liquid handling
• High sensitivity of detection even from low concentrated samples
• User friendly GUI, software, complete sample traceability
• Easy maintenance and serviceability
• UV lamp for daily decontamination
• Quick run setup, easy monitoring of progress and powerful analysis tools of BioRad CFX 96TM

Real-Time PCR Kits

Fast and Easy-to-use Tests

Gentaur Kits have been optimized with intense research and development. Instead of risking your tests, it is sufficient to follow this easy procedure for successful reactions and accurate results. It is possible to monitor the nucleic acid amount on-line, during the reaction utilizing hydrolysis probe method in which fluorescence emission increases proportionally to the DNA amount.

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Features

●     Ready-to-use system for pathogen detection

●     High specificity with perfectly-designed oligos

●     High sensitivity with special reaction mixtures

●     Very reliable results by Internal Control, which is added at the beginning of the extraction process and takes place until the end of the PCR process

●     For pathogen quantification with high reliability, international standards of the World Health Organization (WHO) are used during calibration of RTA External Controls.

●     In compliance with European Union’s directive of in vitro diagnostics products (98/79/EC)


HBV Real-Time PCR Kit

Gentaur HBV Real-Time PCR Kit is an in vitro nucleic acid amplification system used for quantitation of Hepatitis B Virus (HBV) DNA in human serum or plasma. This kit is designed to be used in the treatment of chronic HBV-infected patients having an anti-viral therapy to assess the response with all related clinical and laboratory findings. Gentaur HBV Real-Time PCR Kits use external standards to obtain quantitative results and also contain an internal control to check the DNA isolation and amplification.

Catalog No

Test No

09011025

25 Tests

09011100

100 Tests

HCV Real-Time PCR Kit

Gentaur HCV Real-Time PCR Kit is an in vitro nucleic acid amplification system used for quantitation of Hepatitis C Virus (HCV) RNA in human serum or plasma. It is a one step real time reverse transcription PCR assay in which RNA templates are first reverse-transcribed to generate complementary cDNA strands followed by DNA polymerase-mediated cDNA amplification. This kit is designed to be used in the treatment of chronic HCV-infected patients having an anti-viral therapy to assess the response with all related clinical and laboratory findings. Gentaur HCV Real-Time PCR Kits use external controls to obtain quantitative results and also contain an internal control to check the RNA isolation and amplification.

Catalog No

Test No

09012025

25 Tests

09012100

100 Tests

HIV-1 Real-Time PCR Kit

Gentaur HIV-1 Real-Time PCR Kit is an in vitro nucleic acid amplification system used for quantitation of HIV Type-1 Viral RNA in human serum or plasma. It is a one step real time reverse transcription PCR assay in which RNA templates are first reverse-transcribed to generate complementary cDNA strands followed by DNA polymerase-mediated cDNA amplification. This kit is designed to be used in the treatment of chronic HIV-1-infected patients having an anti-viral therapy to assess the response with all related clinical and laboratory findings. Gentaur HIV-1 Real-Time PCR Kits use external controls to obtain quantitative results and also contain an internal control to check the RNA isolation and amplification.

 

Catalog No

Test No

09013025

25 Tests

09013100

100 Tests

CMV Real-Time PCR Kit

 Gentaur CMV Real-Time PCR Kit is an in vitro nucleic acid amplification system used for quantitation of Cytomegaloviruses (CMV) DNA in human serum or plasma. This kit is designed to be used in the treatment of chronic CMV-infected patients having an anti-viral therapy to assess the response with all related clinical and laboratory findings. Gentaur CMV Real-Time PCR Kits use external controls to obtain quantitative results and also contain an internal control to check the DNA isolation and amplification.

Catalog No

Test No

09014025

25 Tests

09014100

100 Tests

 

Real-Time PCR Instruments

BIO-RAD CFX96™

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The six-channel system combines advanced optical technology with precise thermal control to deliver sensitive, reliable detection. Quickly set up runs and monitor amplification traces in real time on the integrated LCD touch screen. With up to five-target detection, unsurpassed thermal cycler performance, unrivaled stand-alone functionality, and powerful yet easy-to-use software, the CFX96 Touch system is designed to advance your qPCR.

Key Features and Benefits

With the CFX96 Touch Real-time PCR detection system you can:

  • Set up your system quickly — easy installation and factory-calibrated optics
  • Minimize sample and reagent use — up to 5-target multiplexing with sample volumes as low as 10 µl
  • Optimize reactions in a single run — thermal gradient feature
  • Analyze data faster — visualize all run data at once and export only the data you need in the format you want
  • Use advanced data analysis tools — normalized gene expression using CFX Manager™ software
  • Configure the system to fit your needs — run without a computer, run up to 4 systems from 1 computer, or integrate with the CFX automation system for higher throughput

 

INCEPTRA 9660

With Inceptra, product of advanced technology, the user will find it easy to perform both routine diagnostics tests and research.

Key Features and Benefits

●   Inceptra® has a broad linear range. It can detect fluorescence up to 10 orders of start DNA copies without the need for gradient dilution.

●   Automatic Hot-lid technology ensures smooth polymerase chain reaction even in test tubes with different heights; and prevents evaporation of reagents by applying constant pressure.

●   The adopted fluorescent real-time detection mode realizes simultaneous amplification and detection in the same tube without any need for post- treatment. Consequently it is possible to monitor the reaction on-line and intervene when necessary.

●   As a light source for excitation, it uses maintenance-free LED lamp of long service life. 

● Advanced thermoelectric refrigeration technology ensures fast and steady heating and refrigerating of ultra-fast heat cycle system.

●   SOAK Low Temperature Conservation function

●   Features the following functions: Absolute Quantification, Relative Quantification, SNP Analysis, Melting Curve, Gradient

●  Also variable about socket alternatives: RS232C Adapter or optionally USB adapter and Blue Tooth adapters are available.

Nucleic Acid Isolation Kits

Genomic DNA Isolation Kit from Tissue

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Gentaur Genomic DNA Isolation Kit from Tissue provides a rapid and easy method for isolation of pure genomic DNA from tissues and paraffin-embedded tissues. In contrast to the laborious and time-consuming traditional methods employing hazardous organic solvents, the kit follows a simple, efficient and fast methodology. Containing genomic DNA specific spin columns and solutions with specially optimized compositions, high quality genomic DNA with high yield is obtained at the end of the procedure. The kit is efficient in extracting genomic DNA from all kinds of solid tissues.

Downstream Applications: The purified genomic DNA is suitable for use in downstream applications such as PCR, Real-Time PCR, Southern Blot, Restriction Enzyme Digestion, RFLP analysis, Hybridization studies, SNP analyses, Genotyping, or Sequencing.

Format

Spin column

Sample

Tissue or paraffin-embedded tissue

Yield

Variable according to the tissue source

Purity

A 260 / A 280 ratio: 1, 80 – 1,90

Speed

Total processing time depends on the tissue source.

Catalog No

Test Size

09003050

50 test

09003100

100 test

Genomic DNA Isolation Kit from Blood

Gentaur Genomic DNA Isolation Kit from Blood provides a fast and easy method for obtaining high purity chromosomal DNA from blood samples by using genomic DNA specific spin columns and solutions with optimized composition. In contrast to the laborious and time-consuming traditional methods employing hazardous organic solvents, the kit follows a simple, economical and fast methodology.

Downstream Applications: The purified genomic DNA is suitable for use in downstream applications such as PCR, Real-Time PCR, Southern Blot, Restriction Enzyme Digestion, RFLP analysis, Hybridization studies, SNP analyses, Genotyping, or Sequencing.

Format

Spin column

Sample

Fresh blood or frozen blood preserved with citrate, heparin or EDTA

Yield

6 µg DNA from 200 µl blood sample

Purity

A 260 / A 280 ratio: 1,80 – 1,90

Speed

About 25 minutes

Catalog No

Test Size

09001050

50 test

09001100

100 test

Viral NA İsolation Kit

Isolation of high-purity viral nucleic acid from serum and plasma samples is essential for clinical diagnosis and molecular research. Gentaur Viral Nucleic Acid Isolation Kit provides a rapid and simplemethod for isolation of highly pure viral nucleic acid from virus containing cell-free body fluids. Due to the simple procedure of the kit that requires only basic laboratory equipment, it is possible to isolate viral nucleic acids from various samples simultaneously.

Downstream Applications: The recovered viral nucleic acids can be used directly in downstream applications such as RT-PCR, qRT-PCR, qPCR, viral detection, viral load determination and viralgenotyping.

Format

Spin column

Sample

500 μl Serum or plasma

Yield

Variable according to viral load

Purity

A 260 / A 280 ratio: 1, 80 – 1,90

Speed

About 25 minutes

Catalog No

Test Size

09029050

50 test

09029100

100 test

Viral DNA Isolation Kit

Some applications of nucleic acid detection require very sensitive techniques while the efficiency, in terms of recovery rate of extraction and purification of nucleic acid, becomes crucial. For example, PCR has made possible the rapid laboratory diagnosis and immediate management of some viral infections. Viruses are often present in very low concentrations in biological fluids, such as serum or plasma.  Gentaur Viral DNA Isolation Kit enables isolation of high quality DNA from viruses in serum or plasma samples, offers a high degree of efficiency, and is practical as well as affordable for use in clinical diagnostic laboratories.

Downstream Applications: The purified viral DNA is suitable for use in downstream applications such as PCR, Real-Time PCR, Southern Blot, Hybridization studies, Genotyping or Sequencing.

Format

Spin column

Sample

Serum or plasma

Yield

Variable according to viral load

Purity

A 260 / A 280 ratio: 1, 80 – 1,90

Speed

About 25 minutes

Catalog No

Test Size

09006050

50 test

09006100

100 test

Viral RNA İzolasyon Kit

Isolation of high-purity viral RNA from serum and plasma samples is essential for clinical diagnosisand molecular research. Gentaur Viral RNA Isolation Kit provides a rapid and simple method for isolationof highly pure viral RNA from virus containing cell-free body fluids. High efficiency and purity isachieved by the end of the isolation process. Due to the simple procedure of the kit that requires only basic laboratory equipment, it is possible to isolate viral RNA from various samples simultaneously.

Downstream Applications: The recovered viral RNA can be used directly in downstream applications such as RTPCR, qRT-PCR, qPCR, viral detection, viral load determination and viral genotyping.

Format

Spin column

Sample

150 μl Serum or plasma

Yield

Variable according to viral load

Speed

About 25 minutes

Catalog No

Test Size

09010050

50 test

09010100

100 test