Fluorescent detection of vimentin and lamin IHC B1 in normal colon tissue. Sections of normal human colon cancer tissues were blocked with BSA buffer Thermo Scientific blocking windows before incubation with anti-vimentin antibodies and anti-lamin B1 antibody, followed by incubation with Thermo Scientific DyLight 405 goat anti-mouse or Thermo Scientific DyLight 549 goat anti-rabbit secondary antibodies, respectively. The cell nuclei were against green.
Introduction